Western blot analysis of ATP5G1,2,3 in, Lane 1: human HEPG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human U937 whole cell lysates, Lane 7: human CACO-2 whole cell lysates, Lane 8 :rat kidney tissue lysates, Lane 9: rat stomach tissue lysates, Lane 10: rat liver tissue lysates, Lane 11: mouse kidney tissue lysates, Lane 12: mouse stomach tissue lysates, Lane 13: mouse liver tissue lysates, Lane 14: mouse lung tissue lysates, Lane 15: mouse Neuro-2a whole cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with ATP5G1/2/3 Polyclonal Antibody (Product # PA5-114397) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0. 1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for ATP5G1,2,3 at approximately 10-14KD. The expected band size for ATP5G1,2,3 is at 10-14KD.